g-quadruplex structures are stable and detectable in human genomic dna pdf

G-quadruplex structures are stable and detectable in human genomic dna pdf

File Name: g-quadruplex structures are stable and detectable in human genomic dna .zip
Size: 16257Kb
Published: 02.06.2021

We apologize for the inconvenience...

Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons

Background

Protocol DOI: G-rich DNA sequences can form four-stranded G-quadruplex G4 secondary structures and are linked to fundamental biological processes such as transcription, replication and telomere maintenance. G4s are also implicated in promoting genome.

We apologize for the inconvenience...

The formation and stabilization of DNA G-quadruplexes in the human telomeric sequence have been shown to inhibit the activity of telomerase, thus the telomeric DNA G-quadruplex has been considered as an attractive target for cancer therapeutic intervention. However, knowledge of the intact human telomeric G-quadruplex structure s formed under physiological conditions is a prerequisite for structure-based rational drug design. This telomeric G-quadruplex structure contains three G-tetrads with mixed G-arrangements, which are connected consecutively with a double-chain-reversal side loop and two lateral loops, each consisting of three nucleotides TTA. This hybrid-type G-quadruplex topology suggests a straightforward pathway for the secondary structure formation with effective packing within the extended human telomeric DNA. The hybrid-type telomeric G-quadruplex is most likely to be of pharmacological relevance, and the distinct folding topology of this G-quadruplex suggests that it can be specifically targeted by G-quadruplex interactive small molecule drugs. Telomeres are non-coding highly repetitive sequences at the ends of chromosomes that provide protection against gene erosion at cell divisions, chromosomal non-homologous end-joinings and nuclease attacks 1 — 3. Each replication results in a 50— base loss of the telomere.

RNA G-quadruplex rG4 secondary structures are proposed to play key roles in fundamental biological processes that include the modulation of transcriptional, co-transcriptional, and posttranscriptional events. Recent methodological developments that include predictive algorithms and structure-based sequencing have enabled the detection and mapping of rG4 structures on a transcriptome-wide scale at high sensitivity and resolution. The data generated by these studies provide valuable insights into the potentially diverse roles of rG4s in biology and open up a number of mechanistic hypotheses. Herein we highlight these methodologies and discuss the associated findings in relation to rG4-related biological mechanisms. In Bang reported that guanylic acid GMP could form a gel, suggestive of a self-assembly phenomenon Bang

Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons

These metrics are regularly updated to reflect usage leading up to the last few days. Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts. The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric. Find more information on the Altmetric Attention Score and how the score is calculated. G-quadruplexes are higher order DNA structures that play significant roles in gene transcription and telomeric maintenance.


On the other hand, G-quadruplex formation in telomeric DNA, from human genomic DNA (Figure 1c) [37]. involving four tracts of Gs; the structures detected with G4-specific antibodies are presumed to the ability of G-rich telomere repeats to form stable secondary structures that confers an evolutionary.


Background

To date, only G4s with all guanines originating from the same strand of DNA have been considered in the context of the human nuclear genome. Here, I discuss interstrand topological configurations of G-quadruplex DNA, consisting of guanines from both strands of genomic DNA; an algorithm is presented for predicting such structures. I have identified over , non-overlapping interstrand G-quadruplex forming sequences in the human genome—significantly more than intrastrand configurations. Functional analysis of interstrand G-quadruplex sites shows strong association with transcription initiation, the results are consistent with the XPB and XPD transcriptional helicases binding only to G-quadruplex DNA with interstrand topology.

Metrics details. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. However, there is a lack of direct evidence for RNA G-quadruplex formation in living eukaryotic cells. Therefore, it is unclear whether any purported functions are associated with the specific sequence content or the formation of an RNA G-quadruplex structure.

3 comments

  • Peppin D. 04.06.2021 at 00:10

    Jazz guitar chord chart with finger position pdf john deere x485 service manual pdf

    Reply
  • Azura P. 04.06.2021 at 10:53

    Brand management in pharma ppt to pdf thanks for the memories cecilia ahern pdf

    Reply
  • Bradley O. 09.06.2021 at 10:03

    These results confirm the existence of G-quadruplex structures and their persistence in human genomic DNA. Guanine-rich DNA can form.

    Reply

Leave a reply